Cov khoom

Product Detail:

Innovita® 2019-nCoV IgM/IgG Test is aimed for the diagnosis and epidemiological monitoring of diseases because of the novel coronavirus (2019-nCoV).ORF1ab thiab N gene ntawm 2019-nCoV raug kuaj pom zoo los ntawm caj pas swabs thiab cov kua dej alveolar lavage sau los ntawm cov neeg mob uas xav tias mob ntsws, cov neeg mob uas xav tias muaj mob, lwm tus uas yuav tsum tau kuaj mob.
Cov txiaj ntsig ntawm cov khoom siv no tsuas yog siv rau kev kho mob nkaus xwb.Nws raug nquahu kom ua qhov kev ntsuam xyuas dav dav ntawm tus mob raws li tus neeg mob qhov kev kuaj mob tshwm sim thiab lwm yam kev kuaj mob.

Txoj Cai:

Cov khoom siv no siv ib-kauj ruam rov qab hloov pauv polymerase saw cov tshuaj tiv thaiv (RT-PCR) Kev tshawb nrhiav thev naus laus zis txhawm rau txhawm rau tsom tus kab mob tshiab coronavirus (2019-nCoV) ORF1ab gene, N gene, thiab human internal reference gene sequence.Cov primers tshwj xeeb thiab taqman sojntsuam tau tsim nyob rau hauv thaj chaw khaws cia.

Muaj pes tsawg leeg:

Lus Cim: 1. Cov khoom sib txawv ntawm cov reagents yuav tsum tsis txhob sib xyaw.
2. Kev tswj zoo thiab kev tswj xyuas zoo yuav tsum tsis txhob muab rho tawm

Cov txheej txheem xeem:

1. Nucleic acid extraction:
Kev lag luam RNA cov khoom siv rho tawm yog muaj, cov khoom siv sib nqus cov hlaws rho tawm thiab cov kab sib nqus rho tawm tau pom zoo rau cov khoom siv no.
2. Kev npaj cov tshuaj tiv thaiv Mix:

● Tshem tawm cov tshuaj tiv thaiv 2019-nCoV Mix A/B thiab khaws cia hauv chav sov kom txog thaum tsis khov;
● Siv cov khoom sib txuas (Reaction Mix A 16.5μL/T, Reaction Mix B 3.5μL/T) thiab sib tov, thiab tom qab ntawd aliquot txhua cov tshuaj tiv thaiv PCR nrog 20μL / raj;
● Ntxiv 5μL ntawm RNA template los yog kev tswj tsis zoo los yog kev tswj zoo, ces npog lub raj cap;
● Muab cov tshuaj tiv thaiv raj rau hauv cov khoom siv fluorescence PCR, thiab teeb tsa cov kev tswj tsis zoo / zoo thiab cov qauv tsis zoo rau cov tshuaj tiv thaiv RT-PCR raws li cov lus qhia ua haujlwm.
● Sau cov qauv xaj xaj

3.RT-PCR raws tu qauv:

Cov chaw pom zoo: