Rotavirus/Adenovirus/Norovirus Ag Test
Npaj siv
Cov khoom siv yog npaj rau kev tshawb nrhiav ncaj qha thiab zoo ntawm pawg A rotavirus antigens, adenovirus antigens 40 thiab 41, norovirus (GI) thiab norovirus (GII) antigens hauv tib neeg cov quav quav.
Qhov kev kuaj pom zoo yuav tsum muaj kev pom zoo ntxiv.Qhov kev kuaj pom tsis zoo tsis txiav txim qhov muaj feem kis tau.
Cov txiaj ntsig ntawm cov khoom siv no tsuas yog siv rau kev kho mob nkaus xwb.Nws raug nquahu kom ua qhov kev ntsuam xyuas dav dav ntawm tus mob raws li tus neeg mob qhov kev kuaj mob tshwm sim thiab lwm yam kev kuaj mob.
Cov ntsiab lus
Rotavirus (RV)yog ib yam kab mob tseem ceeb uas ua rau muaj kab mob raws plab thiab enteritis hauv cov menyuam mos thiab cov menyuam yaus thoob ntiaj teb.Lub ncov ntawm qhov tshwm sim yog nyob rau lub caij nplooj zeeg, tseem hu ua "Autumn raws plab ntawm cov menyuam mos thiab cov menyuam yaus".Qhov tshwm sim ntawm cov kab mob kis hauv cov menyuam mos hauv lub hlis thiab hnub nyoog 2 xyoos yog siab li 62%, thiab lub sijhawm incubation yog 1 txog 7 hnub, feem ntau tsawg dua 48 teev, tshwm sim los ntawm qhov mob plab thiab lub cev qhuav dej.Tom qab invading tib neeg lub cev, nws replicates nyob rau hauv lub villous epithelial hlwb ntawm cov hnyuv me thiab yog tso tawm nyob rau hauv loj qhov ntau nrog quav.
Adenovirus (ADV)yog ib tug kab mob DNA ob leeg stranded nrog ib txoj kab uas hla ntawm 70-90nm.Nws yog tus kab mob icosahedral kab mob uas tsis muaj lub hnab ntawv.Cov kab mob feem ntau yog tsim los ntawm cov plhaub protein thiab cov tub ntxhais kawm ob npaug ntawm DNA.Enteric adenovirus type 40 thiab type 41 ntawm pawg F yog cov kab mob tseem ceeb ntawm cov kab mob raws plab hauv tib neeg, feem ntau cuam tshuam rau cov menyuam mos thiab cov menyuam yaus (qis dua 4 xyoos).Lub sij hawm incubation yog li 3 mus rau 10 hnub.Nws replicates nyob rau hauv cov hnyuv hlwb thiab tawm hauv cov quav rau 10 hnub.Clinical manifestations yog mob plab, raws plab, dej quav, nrog rau kub taub hau thiab ntuav.
Norovirus (NoV)belongs rau tsev neeg caliciviridae thiab muaj 20-hedral hais nrog ib txoj kab uas hla ntawm 27-35 nm thiab tsis muaj lub hnab ntawv.Norovirus yog ib qho ntawm cov kab mob tseem ceeb ua rau tsis muaj kab mob ua rau mob plab tam sim no.Tus kab mob no kis tau zoo heev thiab kis tau los ntawm cov dej tsis huv, khoom noj, kev sib cuag thiab aerosol tsim los ntawm cov pa phem.Norovirus yog tus kab mob tseem ceeb thib ob uas ua rau muaj kab mob raws plab hauv cov menyuam yaus, thiab nws tawg tawm hauv qhov chaw muaj neeg coob coob.Noroviruses feem ntau muab faib ua tsib genomes (GI, GII, GIII, GIV thiab GV), thiab cov kab mob tseem ceeb ntawm tib neeg yog GI, GII thiab GIV, cov GII genome yog cov kab mob ntau tshaj plaws thoob ntiaj teb.Cov kev kuaj mob lossis chaw kuaj mob ntawm tus kab mob norovirus feem ntau suav nrog electron microscopy, molecular biology thiab immunological detection.
Kev sib xyaw
Kev sau thiab tuav
1. Muab cov quav tso quav tso rau hauv ib lub thawv huv, qhuav.
2. Qhib cov cuab yeej sau cov quav los ntawm unscrewing sab saum toj thiab siv cov shovel sau rau random
3. tho cov quav hauv 2 ~ 5 qhov chaw sib txawv kom sau ib ncig ntawm 100mg cov quav (sib npaug rau 1/2 ntawm cov taum) lossis 100μL kua quav.Tsis txhob muab cov quav tso quav vim qhov no yuav ua rau qhov kev xeem tsis raug.
4. Xyuas kom tseeb tias cov quav ciab tsuas yog nyob rau hauv qhov grooves ntawm lub shovel sau.Cov quav quav ntau dhau tuaj yeem ua rau qhov kev sim tsis raug.
5. Ntsia rau thiab nruj lub hau rau ntawm cov khoom ntim khoom.
6. Caw cov cuab yeej sau cov quav kom huv si.
Cov txheej txheem xeem
1. Nqa cov qauv thiab kuaj cov khoom rau hauv chav tsev yog tias tso rau hauv tub yees lossis khov.
2. Thaum koj npaj txhij pib sim, qhib lub hnab kaw los ntawm kev rhuav tshem ntawm qhov ntsuas.Tshem tawm qhov kev xeem ntawm lub hnab.
3. Muab cov cuab yeej xeem tso rau ntawm qhov chaw huv, tiaj tus.
4. Muab cov khoom ntim cov quav tso ncaj ncaj thiab twist tawm ntawm lub taub hau.
5. Tuav cov khoom ntim cov quav vertically, siv 80μL (ib ncig 2 tee) ntawm cov tshuaj rau hauv cov qauv zoo ntawm cov cuab yeej kuaj.Tsis txhob overload cov qauv.
6. Nyeem cov ntawv xeem tsis pub dhau 15 feeb.Tsis txhob nyeem qhov tshwm sim tom qab 15 feeb.
Cov ntsiab lus txhais
1. Zoo:Lub xub ntiag ntawm ob kab liab liab (T thiab C) nyob rau hauv lub qhov rais tshwm sim qhia tau hais tias zoo rau RV / ADV / NoV antigen.
2. Tsis zoo:Tsuas yog ib kab liab-ntsuab liab tshwm ntawm kab tswj (C) qhia qhov tshwm sim tsis zoo.
3. Invalid:Yog tias cov kab tswj (C) tsis tshwm sim, txawm tias T kab puas pom lossis tsis pom, qhov kev xeem tsis raug.Txheeb xyuas cov txheej txheem thiab rov ua qhov kev sim nrog ib qho kev sim tshiab.
